This project is a literature study with the hypothesis that Arctic climate has a positive effect on the accumulation of metabolites in berries. Plant metabolites are often divided into primary and secondary metabolites. Primary metabolites such as proteins and nucleic acids are essential for cell function, while secondary metabolites are often non-essential, but have important functions regarding reproduction and survival. The berries mentioned in this project are from the families Ericaceae, Rosaceae, Elaeagnaceae och Grossulariaceae.The conclusion drawn from this literature study is that it is not obvious what favours accumulation of metabolites in berries. Other factors aside from climate affect the production of secondary metabolites, such as time of harvest, cultivars and ripeness.

SammanfattningMykotoxiner är sekundära metaboliter som kan bildas av vissa mögelsvampar. Idisslare har generellt ett högre skydd mot mykotoxiner än vad enkelmagade djur har då våmmens mikroorganismer kan fungera som ett extra skydd mot mykotoxikos. Trichotecenerna deoxynivalenol och T-2 toxin bryts ner till de-epoxymetaboliter i våmmen genom att dess toxiska epoxyring spjälkas bort. Deoxynivalenol metaboliseras helt eller delvis av våmmens mikroorganismer, medan T-2 toxinets nedbrytningskapacitet inte är lika hög. Aflatoxin B1 har en låg nedbrytbarhet, och inga metaboliter har kunnat detekteras i våmmen.

The present study discusses the metabolism of ethanol in the human body from the ingestion of ethanol to the excretion of its break down products water and carbon dioxide. Ethanol is a small molecule, soluble in water as well as in organic solutions. It is quickly distributed to every section in the body, where it exerts a direct toxic effect on the cells. Ethanol cannot directly leave the body efficiently so it needs other metabolic pathways. The molecule is metabolized by oxidation, predominately in the liver.

During the last decades the fertility of dairy cows has declined in the same rate as the milk yield has increased. This is a result of the negative genetic correlation between milk yield and fertility, failure to show estrous signs and metabolic problems in the cow. The purpose of this review was to investigate the function of metabolites and hormones as markers for disturbances in fertility, health and production in the dairy cow. There are several metabolites that are suggested to indicate the cow?s energy balance.

The purpose of this study was to evaluate enantioselective analytical methods by separation of the enantiomers of four drugs (citalopram, zopiclone, tramadol and methylphenidate) and their metabolites. The analyses were performed with HPLC-UV with columns whose stationary phases were based on macrocyclic glycopeptides (Chirobiotic V, V2 and T) and polysaccharides (Lux Cellulose-1, Cellulose-2 and Amylose-2).The Chirobiotic V column showed high selectivity for citalopram and its metabolites. High resolution was obtained using a mobile phase consisting of methanol, acetic acid and ammonia. High selectivity for the enantiomers of zopiclone and its metabolites were obtained on the Cellulose-2 column using a mobile phase consisting of acetonitrile and ammonium acetate buffer.The enantiomers of tramadol were separated with the Amylose-2 column. However, changes in the pressure arose, probably caused by the additive NH4HCO3.

The use of intramuscular injections of procaine penicillin G (bensylpenicillin procaine) in the horse is now and then associated with acute adverse reactions (penicillin-chock). The etiology is not yet clearly understood, but the theories are several. One possibility is that it can be caused by procaine toxicity. Procaine penicillin G is a salt which is quickly dissolved in plasma. Procaine is then metabolized by plasmaesterases to non-Toxic metabolites, p-aminobenzoic acid (PABA) and diethylaminoethanol.

A research of 14 toxic affected study sites in Sweden showed a significant increased proportion of deformed diatoms. The six metal polluted study sites in the region of Dalarna showed a 4, 2% average of deformed Fragilaria (deformed diatoms reference sites, 0, 1%). The results also indicate a significant increased proportions of deformed diatoms in the eight pesticide polluted sites in the south and middle of Sweden, compared to the reference sites, howsoever the toxic content was low. The results indicate that there are no lager differences in neither teratological forms nor variations that segregate metal polluted and pesticide polluted shells. One of the reasons may be that both heavy metals and pesticides can affect the development of the diatom shell..

Botanically diverse pastures are commonly used in New Zealand to reduce the ruminant environmental impact by reducing the methane production from the rumen digestion. In order to evaluate the effects diverse pasture species have on the milk fatty acid profile seventy-two lactating Friesian-Jersey crossbred dairy cows were used in a randomised block design with two replicates of six treatments. Six different pasture mixtures were fed at a daily allowance of approximately 15 kg DM/cow/day. The mixtures were categorised as either a simple or a diverse pasture. The simple mixtures all contained white clover with the addition of either a standard diploid perennial ryegrass (RG), a diploid high sugar ryegrass (HS) or tall fescue (TF).

The dairy cow?s ability to resume estrus after calving and to conceive is affected by their metabolic status after calving. For high yielding cows there is a high risk that the feed intake will not be sufficient to cope with the increased energy demands after calving, and the cow will end up in a state of negative energy balance. Energy balance can be measured in various ways, for example by body condition score or by plasma concentration of several metabolic hormones and metabolites. After calving, endocrine pathways stimulate tissue mobilization; insulin signaling is blocked, the concentration of insulin-like growth factor-1(IGF-1) and leptin decreases, and the concentration of growth hormone (GH) increases.

Large quantities of chemicals are used in all areas of society. The chemicals can be toxic and dangerous for the environment if used incorrectly. To protect people and the environment from dangerous chemicals, a good control of them and an active work to phase out hazardous substances in all areas where chemicals are present, are needed. Banverket and Vägverket handle in production a wide range of chemical products. Many of the products are dangerous to the environment, for those who work with them and to others who may come into contact with them.

Important issues for the local authorities are what to do with the sludge produced insewagetreatment plants. The tax for landfill leads to higher costs with 250 SEK/ton. On the 31December 2004 it will be forbidden to landfill sludge.In Sweden the yearly production of sludge is 240 000 tons, counted as DS. The phosphorouscontent is 3 % in sludge. The total yearly production of sludge gives 6000 tons of phosphorous.

Disinfection methods for drinking-water produced at the two water works of Stockholm Water Co are compared in this study. Three different nethods are compared; disinfection with chlorine gas, disinfection with sodium hypochlorite and disinfection with UV-light and monochloramine. The method used is Life cycle assessment, LCA. LCA is defined as the compilation and evaluation of the inputs, outputs and potential environmental impact of a product system shroughout its life cycle. The environmental burden is compared for the three different disinfection methods.

Cats and dogs are often unattended by their owners both indoors and outdoors, and therefore there is always a risk that they will get hurt. In the past, poisoning cases was considered unusual, but nowadays they are considered more common. As a veterinary nurse, it is important to know the common symptoms of poisoning, especially when working with emergency veterinary care. A very essential part of the profession as a veterinary nurse is to inform pet owners about the potentially dangerous substances and how poisonings can be prevented. The purpose of this report was to find out what is written scientifically about some common poisonings in dogs and cats and to present available statistics that covers the number of animals poisoned by various substances in Sweden. Hopefully this essay can also be used as a handbook for professionals in animal health care and to assist in educating pet owners. The report mainly covers groceries found in many homes, often easy accessible.

A growing population together with a change in food habits to a more diverse diet containing more animal products such as meat and milk makes the possibility to produce and sell products of cattle a growing market. And at the same time there is an ongoing debate about global warming, where raising cattle is questioned due to their high production of enteric methane. To be able to raise animals in a more environmental friendly way and at the same time improve productivity in a developing country such as Vietnam would be favorable. The use of feed additives with high electron affinity could decrease the methane production and at the same time improve the gross energy digested. Nitrate and Sulfur are both good electron acceptors, but due to its toxic effects the use of nitrate in feed has been neglected.

The problem of finding targeted medicine is a central problem in chemotherapy. From this point of view the ubiquitin-proteasome system is a highly promising object in the pharmaceutical approach. Proteasome plays a critical role in cellular protein degradation, cell cycle and apoptosis regulation.Proteasome inhibitors are substances blocking the actions of proteasome. Cancer cells are more sensitive to inhibition of the ubiquitin-proteasome system than normal cells. Therefore proteasome inhibitors have the potential to be successfully used in the cancer treatment.The study aimed to test various substances to identify possible proteasome inhibitors with the IncuCyteTM FLR image system and fluorometric microculture cytotoxicity assay.